EasyCluster2: an improved tool for clustering and assembling long transcriptome reads. Conclusions: EasyCluster2 represents a unique tool to cluster and assemble transcriptome reads produced with 454 technology, as well as ESTs and full-length transcripts. The clustering procedure is enhanced with the employment of genome annotations and unspliced reads. Overall, EasyCluster2 is able to perform an effective detection of splicing isoforms, since it can refine exon-exon junctions and explore alternative splicing without known reference transcripts. Results in GFF3 format can be browsed in the UCSC Genome Browser. Therefore, EasyCluster2 is a powerful tool to generate reliable clusters for gene expression studies, facilitating the analysis also to researchers not skilled in bioinformatics.

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  1. Sahlin, Kristoffer; Medvedev, Paul: \textitDenovo clustering of long-read transcriptome data using a greedy, quality-value based algorithm (2019)